FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922816

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922816
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9419329
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA122110.129637684382826No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT95680.10157836083653092No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA17200.045.5590029
CGACCGT12600.040.4773141
ACCGTTG12100.036.9464343
GTGCGCG21650.036.8527648
CCCGTAT27650.030.6848011
CCGTCAT26500.030.217731
GTATCGA28200.029.5133824
GACCGTT16150.029.1536352
AACGTAT6100.028.8470865
ACGTGCG27450.028.7245756
AACACGT27650.028.724433
CGATCGT1004.8411348E-528.5989231
CTATAAC14100.028.0580461
CGCGTAG2053.092282E-1127.901391
CCGTTGA16350.027.3426234
GAACGTA6650.027.1764054
TCGCATA3550.026.7559497
ACGTATT6650.026.4282236
CCGGTAG36750.026.0697671
GACGCTA50500.025.5216054
Produced by FastQC (version 0.11.5)