FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922817

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922817
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9419329
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA117660.12491335635478919No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11050.046.0404241
ACCGTTG11350.041.414743
TGCGCGA17150.036.547519
CTATAAC16300.032.669931
GACCGTT15400.030.8341392
CCGTTGA15900.030.1602764
GTGCGCG21600.030.1172128
CGCGTAG2950.029.0113741
CCGTCAT26550.028.4741271
GAACGTA7450.027.4045854
GTATCGA28700.027.131454
CCCGTAT28850.026.8633161
CCGGTAG32200.025.3974631
AACGTAT7550.025.1553885
TACTCTA31950.024.373679
GACGCTA49350.023.9564934
CTTGGAT57800.023.6086881
TTAAGGC43250.023.4950087
ACGCTAC50800.023.3665315
AACACGT28900.023.1652453
Produced by FastQC (version 0.11.5)