FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922821

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922821
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7272861
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA13550.029.0905869
TAACACG21100.020.511792
GTGCGCG21450.020.147538
ACGTGCG24200.018.056646
AACACGT25900.016.877613
CGCGAGT12250.015.31350810-11
CCGTAAT7300.014.9844461
ACACGTG29850.014.4848484
CTAACAC34500.014.0610181
CGTGCGC32250.013.8440337
CTCGGAT24500.013.2001381
TCGCACG14850.012.31017724-25
CGAGTCG14700.012.1149512-13
CTCGGGT28050.012.038191
GCGCAAT16050.011.98270344-45
CGATTAG11150.011.5055084
CTCGCGT7903.6379788E-1111.4383181
CTCGGTT12300.011.2131751
CTCGACT18250.011.2057591
CACGTGC40200.011.1080545
Produced by FastQC (version 0.11.5)