FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922823

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922823
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6822192
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA11600.023.746149
TAACACG19050.016.951382
ACGTGCG20750.016.4791286
GTGCGCG19300.015.5025488
CGCGAGT9800.014.78076110-11
AACACGT23700.014.2272273
CTCGGAT24450.013.7904041
CTCGGGT24150.013.5684261
CGAAGAT30550.013.0576681
CTCGGTT13850.013.0296091
CTCGCAT19900.012.1706741
CTAACAC30750.012.0461151
CGTGCGC29200.012.0356267
CGACCGT4751.0442654E-611.9973461
CTCGAAT16000.011.8723731
TCGCACG12750.011.54608324-25
CGCGGAT8359.094947E-1211.3747281
ACTCCGC13600.011.1745725
CGAATCG3400.001173511411.1743263
CTCAGAT50800.011.1245071
Produced by FastQC (version 0.11.5)