FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922825

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922825
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6991706
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA12900.020.6158649
TAACACG20900.015.7003572
GTGCGCG19900.014.3186278
ACGTGCG21950.013.8483626
AACACGT24700.013.6565773
CGAAGAT29100.013.5637371
CGCGAGT11400.013.33055910-11
CTAACAC31700.013.0513141
CTCAGAT53900.011.3813881
CGATAAT7551.9463187E-1011.3375651
CTCGACT15150.011.3001481
CTCGCAT21700.010.9573251
CTCGGGT24750.010.9520121
CGACCGT4801.5100817E-510.8979721
CTCGGAT25200.010.7564411
TCGCACG15250.010.74212724-25
CTTTAAT44050.010.6876811
CTCGTAT7652.908564E-910.5677321
TTGAACG14500.010.4817787
CTCGGTT12300.010.4388571
Produced by FastQC (version 0.11.5)