FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922826

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922826
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11375075
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA25450.038.441339
GTGCGCG37800.026.0074338
TAACACG43450.023.5292572
ACGTGCG45600.022.4991976
AACACGT47150.021.9853973
GACGCTA26250.019.9261284
CGCGAGT26600.019.282410-11
CGACCGT8350.018.833971
ACACGTG58250.017.7959064
CGTGCGC59850.017.2192617
CTAACAC67900.017.1953351
ACGCTAC31400.016.8094255
CGAGTCG30950.016.49554812-13
CCCGTAT18250.015.6676091
TCGCACG33600.015.54792124-25
CGCTACT33250.015.1423176
CACGTGC71850.014.9568865
CGTATCG18650.014.7879743
CGATAAC13900.014.7423931
AACGTAG23850.014.155645