FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922832

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922832
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14690701
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGC898240.611434403300428TruSeq Adapter, Index 4 (100% over 50bp)
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT165700.11279243924439004No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA147600.10047172017182843No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG123550.039.22245842-43
CTCGTAT122400.038.25186542-43
TATGCCG124900.038.05665246-47
CGTATGC126400.037.66192244-45
ATCTCGT126700.035.6940340-41
GCCGTCT136850.034.9927148-49
TCGGAAG283350.033.8576623
AAGAGCA291450.033.127177
AATCTCG137850.033.0266438-39
CGTCTTC145600.032.85709850-51
CCGTCTT147750.032.18605450-51
CGACCGT15850.030.938411
TCTCGTA148300.030.68736640-41
GAGCACA320450.030.3204579
AGAGCAC328300.029.7256498
CAGTCAC167200.029.013626-27
ACTGACC167300.028.70663832-33
ATCGGAA335350.028.5902042
CCAATCT160550.028.5479836-37
GATCGGA336000.028.5371231
Produced by FastQC (version 0.11.5)