FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922833

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922833
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14690701
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG902380.6142525125247598Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT149590.101826318567099No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT213500.044.0062648
GCCGTAT119900.038.6309346-47
TGGTCGC122100.037.81410242-43
AAGAGCG259250.036.6985177
TCTCGGT131350.035.7037736-37
TCGGTGG131150.035.68573438-39
GATCTCG133400.035.20705834-35
CTCGGTG134650.035.12853236-37
TCGCCGT132000.034.94586644-45
CAGATTG47950.034.866863
GTAGATC141250.033.93882430-31
TCGGAAG281250.033.775073
CGTATCA137700.033.67197448-49
GAGCGTC287750.032.7170379
ATCTCGG147850.032.39245634-35
GTATCAT146250.031.42542650-51
GTCGCCG149300.031.32610744-45
TAGATCT153300.030.7612932-33
TTGAACG53450.030.4660367
AGAGTGT160900.029.42685926-27
Produced by FastQC (version 0.11.5)