FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922838

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922838
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14697853
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC170520.11601694478778635No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT15200.027.8394151
GTATCGA35850.025.4353624
ACCGTTG15150.024.7650283
TGCGCGA52400.024.4729549
GTGCGCG62550.021.0326088
GCGAACG12400.020.6829627
CCCGTAT47000.019.6253591
AACGTAT14400.019.45916
CGAGCGA13600.019.206564
CCGTCAT29500.019.179561
CGTATCG53050.018.7104953
CGCGTAA3105.529728E-918.404891
TTGAACG43100.018.2924337
ACGTATT15150.018.18267
TGGCGAC47900.017.5691781
TCGAAGG71100.017.4345847
CGCGACT4154.0017767E-1117.185291
ATTGAAC47850.016.5755426
CTCAGAT95200.016.481271
CTATAAC20700.015.8486561
Produced by FastQC (version 0.11.5)