FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922839

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922839
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302820
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT206290.15507238314883612No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA19200.035.6223569
CGCGTAA2550.033.58791
CGACCGT18900.031.7219051
ACCGTTG20050.028.1899283
GTGCGCG26300.027.0892438
CCGGTAG89100.023.7647251
CCGTCAT35850.023.3600641
GTATCGA29650.022.9072384
CTATAAC24150.022.6585031
CGGTAGC94050.022.3720762
TTGAACG37750.022.2698697
GACGCTA41050.021.9837514
ACGCTAC41900.021.6511385
CGATCGT2005.3052463E-721.4122871
CGCTACT44000.020.7257356
GACCGTT28400.020.2361722
TCGAACG20350.020.0722240-41
TAGCGGC106700.019.8977745
AGCGGCC113800.019.1989276
CTCAGAT84500.018.9205231
Produced by FastQC (version 0.11.5)