FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922840

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922840
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13302820
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT153320.11525375822569951No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA21000.033.018629
CGACCGT17950.025.9262431
GTGCGCG28050.025.735618
GACGCTA41350.024.0036074
TTGAACG35300.023.678917
CCGTCAT34900.023.5395771
GTATCGA30500.023.5116394
CTATAAC23400.023.1348651
GAACGTA10350.022.9422174
ACGCTAC43200.022.7564135
ACCGTTG18800.022.4820693
CCGGTAG84500.021.7486481
AACGTAT11200.020.7775945
CGCTACT47500.020.696596
CGGTAGC89400.020.3970992
CGCGAGT20000.019.70940410-11
ACGTGCG42950.019.682446
CTCAGAT85500.019.4392681
AACACGT45250.019.4158883
CGCGTAA2451.7644379E-719.3825951
Produced by FastQC (version 0.11.5)