FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922842

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922842
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13638225
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT142320.10435375571234526No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA20600.030.89079
GTGCGCG27400.024.0909758
CGACCGT18700.023.1404611
CCGGTAG87900.022.8294681
CTATAAC25700.022.2034451
GACGCTA38200.022.0133724
CGGTAGC89450.022.0080242
TTGAACG36050.021.3428557
ACGCTAC41650.020.7572235
ACCGTTG19450.020.5181373
AACGTAT11250.019.8453255
CCGTCAT37900.019.6984771
GTATCGA30100.019.2561744
ACGTGCG41400.018.5847136
TCGAACG20400.018.50580640-41
CGCTACT47800.018.1829516
CGCGTAA3408.8039087E-1018.181791
TAGCGGC105650.018.1195935
GAACGTA11400.017.9200944
CTTGGAT88600.017.6040381
Produced by FastQC (version 0.11.5)