FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922846

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922846
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22891605
Sequences flagged as poor quality0
Sequence length51
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT9350.018.52653145
TCTCGTA9700.018.0952744
CGTGCGA20100.015.888988510
TCGTTGT21050.015.40009734
CATCGTT22100.014.97338732
ATCTCGT12300.014.63977143
TATACCG22350.014.5922726
CGGTATG13350.013.82553743
ACGAGAC14300.013.37719128
GCGCTAT29850.013.33683819
TCGTTCG11500.013.30294823
TATACAC263700.013.2999845
CTCGCTA21800.013.10123713
CGAGAAT23000.013.00464114
CCGTTTA24000.012.46250913
CCGTGCG32150.012.3821559
TTGCGAT13700.011.98378316
GGCGCTA34150.011.85511418
TCCGTAT20000.011.80658813
CGTTTAC25800.011.59328514
Produced by FastQC (version 0.11.5)