FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922847

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922847
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences31530772
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG10000.019.80595843
CGTGCGA23500.019.43162710
CTCGTAT10700.018.30230739
TCGTTGT29000.018.08110434
TCTCGTA11150.017.96741138
CATCGTT30250.017.26010332
TCGTATG12550.016.14243940
TCGTTCG27650.016.02801123
CGTATGC12700.015.59729541
CGTTCGG28900.015.41413924
CGGTAAG29200.015.25901828
TTCGGTA28950.015.23426226
CGAGAAT29050.015.02240114
CGTTCAT26800.014.19432442
CGGTATG17850.014.12189543
GCGAAGT21700.014.10457332
CCGTGCG33050.014.0890369
TCGTTCA28250.013.46600141
TTGCGAT21650.013.09202216
TATACCG38750.013.0035146
Produced by FastQC (version 0.11.5)