FastQCFastQC Report
Fri 3 May 2024
EGAF00004922851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15640168
Total Bases1.5 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT376530.24074549582843355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT272570.17427562159178853TruSeq Adapter, Index 14 (97% over 44bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC234770.15010708324872213No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC175400.11214713294639801No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)