FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922851

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922851
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15640168
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT376530.24074549582843355No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACAGTTCCGTATCTCGTAT272570.17427562159178853TruSeq Adapter, Index 14 (97% over 44bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC234770.15010708324872213No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC175400.11214713294639801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA100000.045.4029929
GTGCGCG113600.040.469118
TTGAACG31100.030.8473287
TGACACG29750.026.500112
GAACGTA12050.026.0125834
ACGTGCG198550.025.9290146
AACACGT177900.024.640663
ACACGTG207350.024.6224354
CACGTGC215750.023.68585
TAACACG186050.023.4336322
AGACGTG46050.023.3080222
CGCGAGT109650.023.1074710-11
AACGTAT14750.022.8608785
ACGTATT14050.022.647756
GTATCGA17050.022.0054024
CGTGCGC236650.021.9150627
CGAGTCG116450.021.69695312-13
CTCAGAT125500.021.2565671
CTAACAC220350.020.544611
ATTGAAC54300.020.2040656
Produced by FastQC (version 0.11.5)