FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922852

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922852
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15640168
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT353610.22609092178549486No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG276710.17692265198174342Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC231110.1477669549329649No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC176120.11260748605769451No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA101550.042.466859
GTGCGCG115600.037.716538
TGACACG31500.028.494752
TTGAACG31550.027.6990817
ACGTGCG205500.024.8221476
ACACGTG213700.023.445614
AACACGT179900.023.0724563
CACGTGC223950.022.5220475
GTATCGA17150.022.430274
TAACACG191950.021.8714562
CGCGAGT112450.021.56157310-11
CGTGCGC237800.021.2908157
TGGCGAC69300.020.764441
CGAGTCG117950.020.555612-13
CTCAGAT132550.020.3148731
CCCGTAT21200.020.1612511
CTAACAC222400.019.7950121
ATCGAAG63050.019.5855226
AGACGTG46000.019.3062252
GAACGTA13350.019.2099444
Produced by FastQC (version 0.11.5)