FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922854

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922854
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7085577
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA12950.026.7715249
TAACACG19350.019.633532
GTGCGCG20000.017.3345628
ACGTGCG22350.017.211856
AACACGT23700.016.4310043
CGACCTA4401.6734703E-915.1105414
CGCGAGT11500.015.07353210-11
CGACCGT5105.638867E-1114.8984891
CTCGGGT28700.014.5610671
CTCGGAT23350.014.439891
CTAACAC32200.014.3056731
ACACGTG30250.013.1873824
CGCCAAC17150.013.014461
CGTGCGC32550.012.2559887
CTCCGAT16050.012.1311281
CGTATCG8750.011.9402443
CGCGAAT4507.49034E-611.6084061
TCGCACG15550.011.60464424-25
CGAGTCG14750.011.43361812-13
CCCGTAT10900.011.3276361
Produced by FastQC (version 0.11.5)