FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922859

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922859
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14758050
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG474600.32158720156118187Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGAGCGT141550.036.002048
AAGAGCG165950.030.708587
CGTATCA82500.030.68852448-49
GCCGTAT84950.029.72043246-47
TGGTCGC90500.027.68746842-43
GAGCGTC186850.027.4521739
CGCCGTA96550.025.9774746-47
TCTCGGT98450.025.95665236-37
TCGGAAG196250.025.893853
GTAGATC104700.025.33449730-31
CGACCGT11650.025.3014051
GATCGGA205500.024.661771
ATCGGAA207000.024.5032372
TCGCCGT105500.024.47151244-45
TCGGTGG105200.024.08747338-39
GTATCAT105350.023.71656450-51
CCGTATC106550.023.47186748-49
CTCGGTG114400.023.14733336-37
GATCTCG110600.023.12613934-35
TGCGCGA30450.022.4605949
Produced by FastQC (version 0.11.5)