FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922862

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922862
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9835542
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC698740.7104234825086406TruSeq Adapter, Index 7 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCAACTCGTATGCC169460.17229350451657874TruSeq Adapter, Index 7 (98% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA155550.074.5530241
TCGGAAG164200.070.2855762
AGAGCAC206950.056.7074857
AGCACAC211750.055.5790339
GAGCACA217200.054.1407058
CGGAAGA215000.053.6122973
AAGAGCA235200.049.270346
GAAGAGC301800.038.6178785
ACACGTC163100.035.67099412-13
ACGTCTG166450.034.82467314-15
TCGTATG159600.034.3360542-43
CTCGTAT159650.034.2796140-41
CACGTCT172600.033.6663712-13
CGTCTGA171350.033.62093414-15
GGAAGAG348450.033.352364
CACACGT177000.032.84288410-11
GCACACG181300.032.54855310-11
TATCGCG18100.030.56894522-23
ACCAGAT183800.029.9223830-31
TCTCGTA147250.029.2458940-41
Produced by FastQC (version 0.11.5)