FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922863

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922863
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9835542
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT161970.16467826582408981Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGAA142050.070.512461
TCGGAAG149100.066.669062
AGAGCGT118050.065.985447
AAGAGCG150700.064.895326
AGCGTCG140050.055.9588669
GAGCGTC147550.052.7928288
CGGAAGA198800.049.858453
AGAGCGG71050.046.2607047
ATCGGGA33900.042.8698771
AGCGGCG86050.039.0796289
GAGCGGC93000.035.4954038
GTAGATC144400.035.0639930-31
GAAGAGC283500.034.930925
CGCCGTA98350.034.5591444-45
GCCGTAT101100.033.80748446-47
CGTATCA99550.033.6423748-49
GCGTCGT102250.033.37578610-11
GATCTCG149200.033.363532-33
CGTGTAG135750.032.887414-15
GAGTGTA138100.031.8814926-27
Produced by FastQC (version 0.11.5)