FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922864

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922864
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14077219
Sequences flagged as poor quality0
Sequence length101
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT155820.11068947638024243No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC144320.10252024920547162No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA51850.029.9499179
TTGAACG27950.023.4488587
GTGCGCG68250.023.3098138
CGACCGT10300.021.2621751
GTATCGA17600.019.1596284
ACCGTTG11000.018.5659473
GAACGTA10800.018.0302374
CCCGTAT22250.017.1177641
CGACCTA4455.456968E-1217.0765974
ATTGAAC47900.016.9546816
AACACGT113950.016.7553353
ACGTGCG116050.016.738146
AACGTAT11650.016.307055
CGCGTAA2457.1987466E-515.5457251
ACGTATT12450.015.2587746
CGCGAGT53250.015.20568710-11
TAACACG132150.014.8496282
CTCAGAT94750.014.5715511
ACACGTG133800.014.3760734
TCGAACG22150.013.93962840-41
Produced by FastQC (version 0.11.5)