FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922868

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922868
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24698923
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT296450.1200254764144979No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA87450.031.9276839
GTGCGCG115650.025.661638
TTGAACG61900.019.177827
GTATCGA38300.019.1061634
CGACCTA9250.019.006914
CGACCGT24200.018.1036971
GAACGTA15000.017.7397824
CGCGAGT88600.017.25726710-11
ACCGTTG23100.017.0733053
AACGTAT15800.016.8415665
AACACGT205600.016.8251843
ACGTGCG210150.016.7883996
TAACACG212550.016.1632522
ATTGAAC87950.015.7651056
CTCAGAT155350.015.5721021
TCGAACG36050.015.28356940-41
CGAGTCG100650.015.14401812-13
ACGTATT16700.015.0698616
CGCGTAA10500.014.9664731
ACACGTG236350.014.7165844
Produced by FastQC (version 0.11.5)