FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922872

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922872
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505251
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT120470.11467598441960121No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA34750.035.2627949
GTGCGCG46550.027.446348
GTATCGA18450.022.1387944
CGACCGT9050.021.0308951
ACGTGCG80350.019.5065256
TAACACG80950.019.3032722
AACACGT80600.019.269243
CGCGAGT36100.019.0113310-11
ACCGTTG10350.018.8146153
CGAGTCG40950.016.75968412-13
GTCTAAC22750.016.5231191
CGTATCG25150.016.4298323
ACACGTG96300.016.3743324
CGTGCGC97550.016.3592667
TTCGCGC10900.016.1223037
TTGAACG22700.016.1107857
CTAACAC107900.016.0518931
CCCGTAT25100.015.9239891
CACGTGC101900.015.474475
TCGCACG47200.014.69138424-25
Produced by FastQC (version 0.11.5)