FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922873

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922873
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10505251
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA32550.032.097959
GTGCGCG41250.026.4793558
GTATCGA15450.021.218194
CGACCGT9750.020.4848161
TAACACG72550.019.0086862
ACGTGCG69600.018.9020656
AACACGT73700.018.5013833
CGCGAGT32550.017.07031410-11
GTCTAAC22300.016.2067571
ACACGTG84350.016.1653294
CGTGCGC83800.016.0391437
CCCGTAT25250.015.8199551
CGTATCG23800.015.7702983
ACCGTTG10300.015.68308453
CGAGTCG37950.014.82858812-13
CACGTGC90650.014.6727575
CTCGGAT36200.014.5814851
CTAACAC99900.014.5660991
CAGACGT24100.014.4043341
TTGAACG20550.014.329097
Produced by FastQC (version 0.11.5)