FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922874

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922874
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20137541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA441030.2190088650843715No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT373210.18533047307017278No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTG97450.049.368963
TTGAACG101950.047.283027
ATTGAAC123200.039.744256
GAACGCT132000.036.5909239
CTCAGAT152650.035.9148981
TCAGATT151400.035.5096932
AACGTAT56950.032.273325
ACGTATT57600.032.0740286
GAACGTA57850.031.8533234
CGACCGT21500.030.7410341
AACGCTG84000.028.18462810-11
AGATTGA192300.028.154774
CCGGTAG144150.027.8400461
TGCGCGA25750.027.665629
CGCGTAA3150.027.1709021
ACCGTTG21150.025.8234253
TATTCAC72350.025.7320219
GTATCGA54300.025.5393244
TGAACGC197600.024.996138
CCGTCAT43150.024.904041
Produced by FastQC (version 0.11.5)