FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922875

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922875
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20137541
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA439220.21811004630605096No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT305930.151920236934589No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATTG99350.044.7030073
TTGAACG103500.043.0499577
ATTGAAC125550.036.77566
CGACCGT20200.034.3295861
GAACGCT132950.033.7637339
CTCAGAT154850.033.1574861
AACGTAT56550.032.9265635
TCAGATT154350.032.527572
GAACGTA57750.032.3246274
ACGTATT57650.032.2995876
CGCGTAA3350.029.7743021
ACCGTTG21300.029.2135723
CGAGCGA45000.027.1277374
GCGAACG44750.026.8557767
CCGGTAG136000.026.3678741
AACGCTG84450.026.15491310-11
AGATTGA196050.024.979564
AGAGCGT53250.024.8882148
CCGTCAT42150.024.678211
CGCAACG35750.024.446972
Produced by FastQC (version 0.11.5)