FastQCFastQC Report
Fri 3 May 2024
EGAF00004922876

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922876
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16089933
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGC572500.35581254440276416TruSeq Adapter, Index 7 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC312590.1942767567770481No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT280820.17453149121254885No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC218340.1356997571089948No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)