FastQCFastQC Report
Mon 6 May 2024
EGAF00004922877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16089933
Total Bases1.6 Gbp
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG583180.3624502351874306Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC294360.18294669095265964No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT259340.16118152884788273No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC233810.14531446464071665No Hit
CCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATG167680.10421423134577379No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)