FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922877

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922877
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16089933
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG583180.3624502351874306Illumina Single End PCR Primer 1 (100% over 50bp)
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC294360.18294669095265964No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT259340.16118152884788273No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC233810.14531446464071665No Hit
CCCACCTCCAAATATCTCATCAACAACCGACTAATCACCACCCAACAATG167680.10421423134577379No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCTA5800.043.4407421
CAGATTG32150.041.5087743
CGACCGT25100.035.6068421
ACCGTTG24250.034.859643
CGCTAGG4000.033.277251
AGAGCGT189050.032.608048
CTATAAC20300.032.5512851
CTCGGTG109950.032.3572336-37
CCGTCAT37300.032.24491
GCCGTAT105150.032.21099546-47
GCAAAAC41300.031.7375642
GTAGATC119800.030.48570430-31
CGCGTAA2550.029.8283461
TGGTCGC102500.029.4746842-43
AGACGTG31050.029.2136842
CAGACGT31350.028.9631081
AACGTAT17400.028.9311545
CGTATCA117300.028.81265348-49
ACGTATT17650.028.7910616
TCGGTGG108150.028.19549638-39
Produced by FastQC (version 0.11.5)