FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922878

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922878
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187820
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC128500.12613100741866268TruSeq Adapter, Index 2 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGC49250.035.2945442
TGGCCCG57050.030.8852395
AGTATGG56400.030.7682531
TATCGCG28650.030.08740222-23
GTACTGG61200.028.1220041
GATGCAT64350.027.3077497
TACTGGA63950.027.1072242
TGCGCGA31050.026.6146249
CTAGTAC17600.025.0959139
TATGGCC71500.025.0419453
GATATCG38250.022.84646820-21
ATCGCGG38200.022.62771822-23
ATGCATC78300.022.624548
GGATGCA77400.022.5194516
GACCTAG28800.022.4274125
GTGCGCG37700.021.7940278
ATATCGC39900.021.72314620-21
TGGATGC81100.021.6677385
ACGTGCG39350.021.4836466
GGATATC44050.019.73049218-19
Produced by FastQC (version 0.11.5)