FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922879

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922879
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10187820
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG132400.1299591080329256Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGGC46900.035.847872
TATCGCG26500.032.34988822-23
AGTATGG54750.030.7080381
TATGGCC55900.030.6710283
TGGCCCG58300.028.757695
GTACTGG62300.027.5964641
GATGCAT62600.027.541847
TGCGCGA31750.026.927099
TACTGGA67000.025.6605932
GGATGCA73800.024.1343466
ATGCATC73700.023.8448668
GATATCG36350.023.58382620-21
ACGTGCG39200.023.3847056
ATCGCGG36800.023.29543722-23
CTAGTAC15200.022.4982919
ATATCGC38550.022.4227320-21
AACACGT43350.021.692473
TGGATGC80900.021.6046455
GTGCGCG41050.021.1737888
GACCTAG26150.020.16045
Produced by FastQC (version 0.11.5)