FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922881

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922881
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13006611
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA178070.13690730044897936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG54550.032.9850927
CAGATTG56650.032.027923
CGACCGT15450.031.0648081
TGCGCGA22700.030.1167589
GTATCGA30050.029.2406854
ACCGTTG15150.028.8430023
ATTGAAC65550.028.5394216
CTCAGAT81750.028.4828931
CCGTCAT42700.025.2623541
GAACGCT72100.025.0877259
AACGTAT24100.024.631065
GAACGTA24350.024.5771684
ACGTATT24150.024.5761896
TCAGATT91050.024.1011262
CCCGTAT39150.022.6978971
GTGCGCG30600.022.1863678
GCGAACG15850.021.8658737
CTATAAC21700.021.4606131
AACGCTG41650.021.03292510-11
CGAGCGA17750.020.0688824
Produced by FastQC (version 0.11.5)