FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922886

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922886
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14925413
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT12900.030.594251
TGCGCGA24050.024.4876489
ACCGTTG15850.021.2753
GACGCTA39900.020.5927184
GTATCGA28650.020.5559484
ACGCTAC41550.019.8892635
TTGAACG46500.019.0998127
GAACGTA13850.018.8605084
CCCGTAT33650.018.652641
TCGAACG24100.017.93484340-41
CGTATCG34750.017.4942633
AACGTAT14950.016.8374045
ATTGAAC59900.016.8092946
CGCTACT51100.016.172196
CTCAGAT105450.016.1431351
GAACGCT60450.015.4778419
CGGGAAC52150.015.3181511
ACGTATT16300.015.1515216
CAATCGG42000.014.9267444
CCGTATC43800.014.5301852
Produced by FastQC (version 0.11.5)