FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922887

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922887
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14925413
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT11900.026.3435121
TGCGCGA25000.022.4203289
GTATCGA29600.020.3798184
GACGCTA38100.020.1966044
CGACCTA6450.019.8834784
ACGCTAC39350.019.5550335
TTGAACG43600.019.1745917
ACCGTTG14150.018.7983573
CCCGTAT33700.018.6046141
GAACGTA13300.018.5712014
TCGAACG22000.017.05695240-41
AACGTAT14250.016.9997925
ACGTATT15000.016.1502346
ATTGAAC58300.015.8878826
CGCTACT49550.015.7217236
CGTATCG40350.015.4211253
GACGTAT8100.015.2471359
CGATAAC34100.014.9041111
CTCAGAT107950.014.7840461
CAATCGG41300.014.4913484
Produced by FastQC (version 0.11.5)