FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922888

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922888
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6989716
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATAAC8000.014.2749471
TGCGCGA10450.014.0898299
CTCAGAT53100.012.9935191
TAACACG14450.012.8190882
CTCGGGT26400.012.4365071
CGAAGAT30350.012.22895051
ACTCCGC13350.012.0964525
CGACCGT5655.4227712E-811.7905181
ATAACGT9050.011.5460773
GTATCGA9053.6379788E-1211.02125554
CGCGGAT9103.6379788E-1210.9807291
ATCGAAG22800.010.8325036
CGTATCG8056.0936145E-1010.6203043
CCGACAT20250.010.5740361
CGACCTA4054.236024E-410.5547474
GACGCTA11450.010.3703854
CCGGGAT21850.010.235281
ACGTGCG19050.010.2223156
CCCGTAT11250.010.1510731
AATCGGG10951.8189894E-129.9764055
Produced by FastQC (version 0.11.5)