FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922889

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922889
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6989716
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT5205.456968E-1215.5254251
TGCGCGA11050.014.6125839
TAACACG14400.012.8614722
CGAAGAT28550.012.4753591
CTCGGGT28900.011.8313021
CGACTAT3400.00117379711.1740081
ATAACGT8651.6370905E-1110.9803173
GTATCGA8258.0035534E-1110.9370624
CGATAAC7401.718945E-910.9097591
AACACGT18700.010.6661753
CTCGCAT20250.010.553231
ACGCTAC12250.010.4673065
GACGCTA10950.010.408744
ACTCCGC14400.010.223665
CTCGGAT26050.010.2088821
CGAAAAT15450.010.1433951
CTCGACT13150.010.1118391
TTCGCTA6202.3298253E-69.957272
CTCAGAT53150.09.9178521
ACGTGCG18650.09.6764296
Produced by FastQC (version 0.11.5)