FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922890

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922890
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709907
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT269490.17154143560493387No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA187400.11928778445346622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT19650.040.6516151
ACCGTTG18900.038.9486123
TGCGCGA24850.037.4586229
AACGTAT17600.035.3492935
GAACGTA18000.034.2999084
ACGTATT18950.032.5803876
GTGCGCG30200.031.6090348
CCGTCAT43650.031.2628611
CTATAAC27600.028.5976311
GACCGTT26450.027.6514022
CCGGTAG101700.027.0700281
CAGATTG39750.026.4044593
CCCGTAT53500.026.1291081
GTATCGA53450.026.0340654
GACGCTA74500.025.7541484
ACGCTAC75550.025.3962155
CCGTTGA28900.025.142924
CGGTAGC110550.024.8308282
TACTCTA56650.024.647319
CGGGAAC45350.024.5340711
Produced by FastQC (version 0.11.5)