FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922891

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922891
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15709907
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT220150.14013450238756983No Hit
CAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGATCA202830.12910961217020572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA26750.041.0208559
CGACCGT19700.039.7825661
GTGCGCG31550.035.683578
ACCGTTG20750.035.253533
CTATAAC28250.032.4499441
CGCGTAG3150.031.6653121
CCGTCAT43400.030.3155271
GAACGTA18100.029.6552244
ACGTATT19450.028.0866746
AACGTAT19600.027.8703925
GACGCTA76550.027.5511134
CAGATTG42650.027.2864483
GTATCGA53350.027.1560614
CGCGTAA1754.973117E-927.1416991
ACGCTAC78200.027.0912765
GACCGTT27600.026.6756922
CCCGTAT54200.026.290391
CCGGTAG87650.025.5779171
CCGTTGA29950.024.7416764
CGCTACT88300.023.9398486
Produced by FastQC (version 0.11.5)