FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922895

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922895
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7369439
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC110520.14997071011782578No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT77090.10460769130458912No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCGCG14550.027.58238222-23
TATGGCC29900.026.8438423
TGCGCGA31500.023.672869
GTATGGC33400.022.4667282
TGGCGAC25400.021.8766771
GATGCAT35800.021.6255027
CTAGTAC8900.020.2794049
AGTATGG36900.020.207031
TACTGGA39600.019.5488662
CGACCGT7600.018.747221
GTACTGG41150.018.581691
ATCGCGG23100.018.09292222-23
ATGCATC43100.018.0729148
ATATCGC22650.017.71848520-21
GATATCG24050.016.98327320-21
GAATTTA70500.016.908843
GGATGCA47350.016.8519786
TAAGCAT68800.016.7066178
GGCGACC35400.016.3676282
TGGCCCG46450.016.3599195
Produced by FastQC (version 0.11.5)