FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922896

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922896
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14034227
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC195370.1392096622065469No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT152310.10852753058647263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCGT10750.035.4577871
CGCGTAA2700.033.528951
TTGAACG35950.030.7844247
ACCGTTG11700.029.6372093
TGCGCGA52600.028.4414949
GTGCGCG61550.024.8459368
GTATCGA21600.023.5302034
ATTGAAC55300.021.473886
CTCAGAT141800.020.9335041
GAACGTA13450.020.1302074
CAGATTG59650.020.0673683
GAACGCT58850.019.2876369
AACGTAT14700.018.7415855
GCGAACG11500.018.5861177
TCAGATT147150.018.37652
AACGCTG31450.017.89473510-11
TCGTATG38500.017.02496342-43
CGGTTCG21500.017.011893
AGACGTG38200.016.9110764
CCCGTAT30400.016.770281
Produced by FastQC (version 0.11.5)