FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922897

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922897
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14034227
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC185110.13189896386883296No Hit
CTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGTTGACTATC142390.10145909710595388No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT142190.10131658836642732No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG36650.035.8957027
CGACCGT11750.027.9198041
TGCGCGA53350.027.4199669
GTATCGA22100.027.2926064
ATTGAAC55300.026.6236176
ACCGTTG11950.025.4357243
GAACGCT56100.024.0439649
GTGCGCG62950.023.6903028
CAGATTG60900.023.005773
CTCAGAT145450.022.097041
AACGTAT14450.021.363075
GAACGTA14400.020.7783784
ACGTATT16300.020.6871766
CCCGTAT30800.020.5306191
TCAGATT147950.020.2235622
TCGAACG32100.019.8285740-41
AACGCTG33400.019.62380610-11
GTCGAAC35650.018.45300738-39
AGTCGAA37300.017.57305138-39
CGAACGG37800.017.5296840-41
Produced by FastQC (version 0.11.5)