FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922898

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922898
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13472634
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC308790.22919794303029387No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC294430.2185392997390117TruSeq Adapter, Index 2 (100% over 50bp)
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC151830.11269511218073615No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT149840.11121804392518939No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT147910.10978551039091539No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA138200.10257830799827265No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG40300.033.7034157
TGCGCGA48950.031.8209469
TCGTATG66700.029.45293842-43
GTGCGCG55450.028.2620948
CAGATTG53900.025.9934463
CGACCGT13850.025.7784731
ATTGAAC56250.025.4132426
CTCAGAT72150.024.7423341
GCCGTCT77450.024.65813448-49
CCGTCAT28600.024.6343651
GTCACCG82350.024.05964928-29
ACCGTTG13650.024.0075053
CAGACGT39450.023.2892781
CTCGTAT71150.023.13701442-43
GAACGTA14800.022.7838574
TATGCCG72350.022.65429946-47
CGTATGC72100.022.6347744-45
AACGTAT14900.022.6309475
ACGTATT15350.022.2761556
TCAGATT72350.022.128942
Produced by FastQC (version 0.11.5)