FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922899

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922899
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13472634
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC292780.21731459490401062No Hit
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG289240.21468704634891736Illumina Single End PCR Primer 1 (100% over 50bp)
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC143450.10647509610963973No Hit
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT142230.10556955677709348No Hit
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT137720.10222203022809051No Hit
CTCAGATTGAACGCTGGCGGCAGGCCTAACACATGCAAGTCGAACGGTAA135810.10080434160090743No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGAACG37450.033.973867
TGCGCGA46750.030.3633639
CGACCGT14150.027.8725721
CGTATCA64300.027.63177348-49
GAACGTA16050.026.9291934
CCGACAT29300.026.9212911
GTGCGCG54600.026.6935318
ATTGAAC53200.025.6132346
ACGTATT16950.025.4897676
CAGATTG53950.025.4431863
AACGTAT17100.025.2693615
ACCGTTG14500.024.5673523
GTAGATC82550.024.13121230-31
CAGACGT40900.024.0493011
CTCAGAT72650.024.0041241
AGAGCGT132950.023.9247158
CTCGGTG79400.023.80640436-37
GCCGTAT80300.022.3920846-47
AGACGTG42550.021.8794352
TCAGATT69900.021.8126012
Produced by FastQC (version 0.11.5)