FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922900

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922900
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11211997
Sequences flagged as poor quality0
Sequence length101
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT406720.3627542889995422No Hit
CCTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCT309420.275972246514158No Hit
CTTAGATGTCCGGGGCTGCACGCGCGCTACACTGACTGGCTCAGCGTGTG207340.18492691355518556No Hit
CTCACCCGGCCCGGACACGGACAGGATTGACAGATTGATAGCTCTTTCTC189860.16933647056808881No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC186760.16657157507266546No Hit
CAACAACACATCATCAGTAGGGTAAAACTAACCTGTCTCACGACGGTCTA170060.15167681546828812No Hit
CCGACATCGAAGGATCAAAAAGCGACGTCGCTATGAACGCTTGGCCGCCA168480.15026761066739494No Hit
CGCGTAACTAGTTAGCATGCCAGAGTCTCGTTCGTTATCGGAATTAACCA160990.14358726638974306No Hit
CAGAAACCTCCCGTGGAGCAGAAGGGCAAAAGCTCGCTTGATCTTGATTT155570.1387531587816158No Hit
CTCGCATTCCACGCCCGGCTCCACGCCAGCGAGCCGGGCTTCTTACCCAT155520.13870856369297993No Hit
CTCAGACAGGCGTAGCCCCGGGAGGAACCCGGGGCCGCAAGTGCGTTCGA146540.13069928577397943No Hit
GTTAGTTTTACCCTACTGATGATGTGTTGTTGCCATGGTAATCCTGCTCA145670.12992333123171546No Hit
CCCAGGCATAGTTCACCATCTTTCGGGTCCTAACACGTGCGCTCGTGCTC143000.1275419534985605No Hit
CTTGTCTCAAAGATTAAGCCATGCATGTCTAAGTACGCACGGCCGGTACA140780.1255619315631283No Hit
CTCGGCTCGTGCGTCGATGAAGAACGCAGCTAGCTGCGAGAATTAATGTG138530.1235551525745146No Hit
CCTCACTAAACCATCCAATCGGTAGTAGCGACGGGCGGTGTGTACAAAGG136810.122021081525441No Hit
CTTAGAGCCAATCCTTATCCCGAAGTTACGGATCCGGCTTGCCGACTTCC134830.12025511601546095No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT129050.11509992376915548No Hit
CTCCAATGGATCCTCGTTAAAGGATTTAAAGTGGACTCATTCCAATTACA124050.11064041490556945No Hit
CTGAATTTAAGCATATTAGTCAGCGGAGGAGAAGAAACTAACCAGGATTC121420.10829471324332321No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA80400.051.2127049
GTGCGCG85500.048.2134558
GGCTCGT35600.041.541044
CTCGGCT50450.034.3838541
TGTCGAG7350.032.995135
TCGTGCG45200.032.6765827
AGGCGTA46100.032.0386438
GTTACGA14250.030.3664134
CGTGCGT48850.029.9433788
GACAGGC58400.028.661365
AACACGT171750.028.5449263
ACACGTG176500.027.9114254
CTCAGAC62550.027.5038971
CTAACAC182900.027.4625741
CAGGCGT55100.027.3226177
TAACACG182400.027.112872
CACGTGC183550.026.7875585
GCGTCGA27800.026.73524710-11
CGTCGAT28400.026.17041612-13
CGTGCGC190850.025.854617
Produced by FastQC (version 0.11.5)