FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922905

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922905
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14132389
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCGGTAGCGGCCCCCGGCGCGCCGGGCCCGGGTCTTCCCGGAGTCGGGTT327070.23143291626065488No Hit
CTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTACTTGT158740.11232354274992006No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA1700.039.158931
GAACGTA13350.034.4984364
CCGGTAG96750.033.862571
ACGTATT13900.033.4774556
AACGTAT14250.033.319285
CAGATTG39300.032.4988983
CCGTCAT31450.031.7504841
CTATAAC18600.031.4444371
CGACCGT20050.030.8305071
GCGAACG11050.029.6502257
CGGTAGC109700.029.5630422
CGAGCGA11100.028.2312364
ACCGTTG19600.027.6158053
GACGCTA52450.026.3424644
TGCGCGA13050.025.8338419
AGCGGCC122300.025.8188196
ACGCTAC54000.025.586435
TTGAACG48350.025.5339557
TACTCTA40900.025.1928679
GTATCGA40750.024.934184
Produced by FastQC (version 0.11.5)