FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922907

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922907
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6088188
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA10450.028.1749279
GTGCGCG16450.018.7642548
ACGTGCG21100.017.5568246
TTGAACG9900.016.3108847
TAACACG22300.015.9940392
CGCGAGT10000.015.43372410-11
AACACGT23900.015.1083583
CGACCGT3354.56644E-614.1957041
CAATCGG7100.013.3836094
ACACGTG28000.013.0657494
CGAGTCG12450.012.96860912-13
CTCAGAT43050.012.5931231
TCGCACG13050.012.55326624-25
TCGAACG5650.012.18628240-41
CGTGCGC30250.012.0892447
CTAACAC35750.011.8390181
CGAACGG6250.011.77615140-41
CTCGGAT16750.011.6404771
CTCGGTT10200.011.1895551
CGCACGA14100.011.11772926-27
Produced by FastQC (version 0.11.5)