FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922914

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922914
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10252108
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT117810.11491295253620035No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA32950.035.3164379
GTGCGCG44150.027.4331478
ACGTGCG76650.018.4039256
CGCGAGT33750.018.36550710-11
TAACACG78350.017.822742
TTGAACG19900.017.66227
GTATCGA16750.017.5809784
AACACGT78500.017.5466633
GAACGTA6250.017.4788974
CGTATCG22300.017.2522753
CGACCGT9550.016.4442541
CCCGTAT22300.016.4319651
AACGTAT7250.016.3782775
CTCGGAT34350.016.3477841
CGAGTCG38050.016.22762112-13
TCGGATT25500.015.64607052
ACACGTG89400.015.4604334
CGTGCGC93100.015.407197
CACGTGC96050.014.6372845
TAGCGTT7500.014.5657478
Produced by FastQC (version 0.11.5)