FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922920

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922920
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences24665746
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA60850.033.170419
TAACACG87700.025.289472
GTGCGCG92050.023.3204618
AACACGT100000.022.4163283
ACGTGCG105750.021.1974746
CTAACAC122400.019.57841
CGTATGC122900.018.2607351
ACACGTG125150.017.9874654
TATGCCG124950.017.9402393
CGCGAGT63250.017.34498610-11
TCGCACG66450.016.58118224-25
CGTGCGC147300.015.9919497
CGAGTCG72100.015.21595412-13
ATGCCGT163000.013.9271684
CACGTGC174000.013.6198955
CTCGGAT74050.013.2914821
CCCGTAT29700.013.2876991
CTCGGGT82550.012.5564691
ACGAAAG93150.012.26181328-29
CGCAATG92950.011.95936944-45
Produced by FastQC (version 0.11.5)