FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004922926

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004922926
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15367545
Sequences flagged as poor quality0
Sequence length101
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT304640.19823595766272364No Hit
CCGGTATTTAGCCTTAGATGGAGTTTACCACCCGCTTTGGGCTGCATTCC157770.1026644138670165No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGA71650.040.698839
GTGCGCG91250.032.373328
CGACCTA15100.031.766854
TTGAACG31700.027.2672827
TAACACG112550.027.090642
AACACGT122200.025.1067813
ACGTGCG123650.024.7739546
CCCGTAT30500.024.319441
GTATCGA25300.024.0280864
GAACGTA16900.022.48194
CGCGAGT75800.020.67637810-11
CTAACAC158350.020.5384121
CGACCGT11350.020.5271681
AACGTAT19250.020.230795
ACACGTG151650.020.1058444
CGAGTCG78800.019.8892712-13
CGTGCGC161300.019.4918637
TACGCAC24450.018.2590739
ATCGAAG53450.018.037576
ACGTATT20850.017.9949556
Produced by FastQC (version 0.11.5)